The scientific activity of Anna El'skaya is mainly focused on fundamental problems of genome expression on the translational level and molecular mechanisms determining its efficiency and accuracy. She is a co-author of the world known discovery of so called "functional tRNA adaptation" to the synthesis of specific proteins as one of the important regulatory factors. Anna El'skaya is among the scientists who were the first to demonstrate a unique structural and functional organization of the translational apparatus of higher eukaryotes determined by the compartmentation of metabolic processes in eukaryotic cells. The tRNA channeling during eukaryotic elongation is one of the "hot points" in the problem of protein synthesis which is in the focus of her recent investigations.

The activity of the Department headed by Anna El'skaya in the field of applied sciences is devoted to the fundamental and technological questions of bioelectronics and creating novel analytical systems - bio- and chemosensors based on various electrochemical transducers and biological material or biomimics. The most important results are obtained in the development of sensors for medicine, biotechnological practice, food industry, and environmental monitoring. Numerous international grants, wide cooperation with advanced laboratories in Japan, France, Germany, Great Britain and Italy, fruitful participation in key scientific meetings can be considered as an evidence of world acknowledgement of priority and importance of the achievements in this field.

Number of publications: more 350.

Telephone: 380 44 526 0749. Fax: 380 44 526 0759. E-mail: elskaya@imbg.org.ua

Main publications:

in the field of protein biosynthesis

1. Lukash, T.O. Turkivska H.V., Negrutskii B.S., El'skaya A.V., Chaperone-like activity of mammalian elongation factor eEF1A: Renaturation of aminoacyl-tRNA synthetases", International Journal of Biochemistry & Cell Biology -2004, V.36 P. 1341-1347
2. T.V. Budkevich, A.A. Timchenko, E.I. Tiktopulo, B.S. Negrutskii, V.F. Shalak, Z.M. Petrushenko, V.L. Aksenov, R. Willumeit, J. Kohlbrecher, I.N.Serdyuk, A.V.El'skaya, Еxtended conformation of mammalian translation elongation factor 1A in solution, Biochemistry. -2003.- 24.- N51. -P.15342-15349.
3. Budkevich, T.V., Timchenko, A. A., Tiktopulo, E.I., Negrutskii, B.S., Shalak, V.F., Petrushenko, Z.M., Aksenov, V.L., Willumeit, R., Kohlbrecher, J., Serdyuk, I.N., El'skaya A.V. Extended conformation of mammalian translation elongation factor 1A in solution // Biochemistry. - 2002. - V. 41. - P. 15342-15349.
4. Petrushenko Z.M., Budkevich T.V., Shalak V.F., Negrutskii B.S., El'skaya A.V. Novel complexes of mammalian translation elongation factor eEF1A*GDP with uncharged tRNA and aminoacyl-tRNA synthetase. Implications for tRNA channeling // Eur. J. Biochem. - 2002. -V.269. - P. 4811-4818.
5. Turkovskaya H.V., Belyanskaya L.L., Kovalenko M.I., El'skaya A.V. Renaturation of rabbit liver aminoacyl-tRNA synthetases by 80S ribosomes // Int. J. Biochem. Cell Biol. - 1999. - V. 31. - P. 759-768.
6. Negrutskii B.S., Shalak V.F., Kerjan P., El'skaya A.V., Mirande M. Functional interaction of mammalian valyl-tRNA synthetase with elongation factor EF-1 in the complex with EF-1H. // Journ.Biol. Chem. - 1999. - V. 274. - P. 4545-4550.
7. Negrutskii B.S., El'skaya A.V. Eukaryotic translation elongation factor 1. Structure, expression, function and possible role in aminoacyl-tRNA channeling // In: Progress in Nucleic Acid Research and Molecular Biology, eds K.Moldave et al., Academic Press, San Diego/ New York/ Boston/ London/ Sydney/ Tokyo/ Toronto. - 1998. - V. 60. - P. 7-78.
8. Petrushenko Z.M., Negrutskii B.S., Ladokhin A.S., Budkevich T.V., Shalak V.F., El'skaya A.V. ,Evidence for the formation of an unusual ternary complex of rabbit liver EF-1а with GDP and deacylated tRNA. -1997.- FEBS Lett. 407, 13-17.
9. Negrutskii B. S., Budkevich T. V., Shalak V. F., Turkovskaya G. V., El'skaya A. V., Rabbit translation elongation factor 1 alpha stimulates the activity of homologous aminoacyl-tRNA synthetase. -1996.- FEBS Lett. 382, 18-20.

in the field of biosensors

1. A.P.Soldatkin, S.V.Dzyadevych, A.V.El'skaya, C.Martelet, N.Jaffrezic-Renault, Pathways for improving potentiometric and conductometric enzymatic biosensors, Encyclopedia of Sensors, American Scientific Publishers, submitted, 2005.
2. A.P.Soldatkin, V.N.Arkhypova, S.V.Dzyadevych, A.V.El'skaya, J.-M.Gravoueille, N.Jaffrezic-Renault, C.Martelet, Analysis of the potato glycoalkaloids by using of enzyme biosensor based on pH-ISFETs, Talanta.- 2005. -66 -P.28-33
3. S.V.Dzyadevych, A.P.Soldatkin, V.N.Arkhypova, A.V.El'skaya, J.-M. Chovelon, C.Georgiou, C.Martelet, N.Jaffrezic-Renault, Early-warning electrochemical biosensor system for the environmental monitoring based on enzyme inhibition effect, Sensors and Actuators B.- 2004.
4. V.N.Arkhypova, S.V.Dzyadevych, A.P.Soldatkin, Y.I.Korpan, A.V.El'skaya, J.-M.Gravoueille, C.Martelet, N.Jaffrezic-Renault, Application of enzyme field effect transistors for fast detection of total glycoalkaloids content in potatoes, Sensors and Actuators B.- 2004.- 103.- P. 416-422.
5. S.V.Dzyadevych, V.N.Arkhypova, C.Martelet, N.Jaffrezic-Renault, J.-M.Chovelon, A.V.El'skaya, A.P.Soldatkin, Potentiometric biosensors based on ISFETs and immobilized cholinesterases, Electroanalysis.- 2004.- 16.- P. 1873-1882.
6. O.A.Biloivan, S.V.Dzyadevych, O.A.Boubryak, A.P.Soldatkin, A.V.El'skaya, Development of enzyme biosensor based on ISFETs for quantitative analysis of serine proteinases, Electroanalysis.- 2004.- 16.- P. 1883-1889
7. Korpan Y.I., Nazarenko E.A., Skryshevskaya I.V., Martelet C., Jaffrezic-Renault N., El'skaya A.V., Potato Glycoalkaloids: True Safety or False Sense of Security?, Trends in Biotechnology.- 2004. -22, N3. -P.147-151
8. S.V.Dzyadevych, V.N.Arkhypova, A.P.Soldatkin, A.V.El'skaya, C.Martelet, N.Jaffrezic-Renault, Enzyme biosensor for tomatine detection in tomatoes, Analytical Letters.- 2004. - 37, No 8.- P. 1611-1624
9. T.A.Sergeyeva, S.A.Piletsky, E.V.Piletska, O.O.Brovko, L.V.Karabanova, L.M.Sergeeva, A.V.El'skaya, A.P.F.Turner, In situ formation of porous molecularly imprinted polymer membranes, Macromolecules. -2003.- 36.- N19. -P. 7352-7357.
10. V.N.Arkhypova, S.V.Dzyadevych, A.P.Soldatkin, A.V.El'skaya, C.Martelet, N.Jaffrezic-Renault, Development and optimisation of biosensors based on pH-sensitive field effect transistor and cholinesterase for sensitive detection of solanaceous glycoalkaloids, Biosensors & Bioelectronics.- 2003.- 18.- P. 1047-1053.
11. S.V.Dzyadevych, A.P.Soldatkin, Y.I.Korpan, V.N.Arkhypova, A.V.El'skaya, J.-M.Chovelon, C.Martelet, N.Jaffrezic-Renault, Biosensors based on enzyme field effect transistors for determination of some substrates and inhibitors, Analytical and Bioanalytical Chemistry.- 2003.- 377.- P. 496-506
12. T.A. Sergeyeva, S.А. Piletsky, A.A. Brovko, A.V. El'skaya, P.J. Warner, A.P.F. Turner, Substance-selective polymer membranes, UK Patent Application, 0216333.5, 01.07.2002.
13. T.A. Sergeyeva, S.A. Piletsky, E.V. Piletska, O.O. Brovko, L.V. Karabanova, L.M. Sergeeva, A.P.F. Turner, A.V. El'skaya, In situ formation of porous molecularly imprinted polymer membranes, Macromolecules, vol. 36, no. 19, pp. 7352-7357, 2003.
14. T.A. Sergeyeva, S.A. Piletsky, E.V. Piletska, O.O. Brovko, L.V. Karabanova, L.M. Sergeeva, A.V.El'skaya, Synthesis of molecularly imprinted polymer membranes for solid-phase extraction of triazine herbicides from aqueous solutions and their investigation // Reports of National Academy of Sciences of Ukraine, no. 6, pp. 170-174, 2003
15. S.V.Dzyadevych, A.P.Soldatkin, Y.I.Korpan, V.N.Arkhypova, A.V.El'skaya, J.-M.Chovelon, C.Martelet, N.Jaffrezic-Renault. Biosensors based on enzyme field effect transistors for determination of some substrates and inhibitors. Analytical and Bioanalytical Chemistry.- 2003.- 377.- P. 496-506.
16. V.N.Arkhypova, S.V.Dzyadevych, A.P.Soldatkin, A.V.El'skaya, C.Martelet, N.Jaffrezic-Renault. Development and optimisation of biosensors based on pH-sensitive field effect transistor and cholinesterase for sensitive detection of solanaceous glycoalkaloids. Biosensors & Bioelectronics.- 2003.- 18.- P. 1047-1053.
17. S.V.Dzyadevych, V.N.Arkhypova, A.V.El'skaya, N.Jaffrezic-Renault, C.Martelet, A.P.Soldatkin. Conductometric enzyme biosensors for substrates or inhibitors analysis. Current Topics in Analytical Chemistry, 2001, 2, P. 179-186.
18. V.N.Arkhypova, S.V.Dzyadevych, A.P.Soldatkin, A.V.El'skaya, N.Jaffrezic-Renault, H.Jaffrezic, C.Martelet. Multibiosensor based on enzyme inhibition analysis for determination of different toxic substances. Talanta, 2001, 55, P. 919-927.
19. S.V.Dzyadevych, V.N.Arkhypova, Y.I.Korpan, A.V.El'skaya, A.P.Soldatkin, N.Jaffrezic-Renault, C.Martelet. Conductometric formaldehyde sensitive biosensor with specifically adapted analytical characteristics. Analytica Chimica Acta, 2001, 445, P. 47-55.
20. Soldatkin A.P., Volotovsky V., El'skaya A.V., Jaffrezic-Renault N, Martelet C. (2000) Improvement of urease based biosensor characteristics using additional layers of charged polymers // Analytica Chimica Acta, V.403, P. 25-29.
21. S.V.Dzyadevich, Y.I.Korpan, V.N.Arkhipova, M.Y.Alesina, C.Martelet, A.V.El'skaya, A.P.Soldatkin. Application of enzyme field-effect transistors for determination of glucose concentrations in blood serum. Biosensors & Bioelectronics, 1999, 14, P. 283-287.
22. T.A.Sergeyeva, S.A.Piletsky, A.A.Brovko, E.A.Slinchenko, L.M.Sergeeva, T.L.Panasyuk, A.V.El'skaya, Conductimetric sensor for atrazine detection based on molecularly imprinted polymer membranes // The Analyst, vol. 124, no.3, pp.331-335, 1999.